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q5 high fidelity dna polymerase  (New England Biolabs)


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    New England Biolabs q5 high fidelity dna polymerase
    Q5 High Fidelity Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 9986 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dna+polymerase/Q5+Hot+Start+High-Fidelity+DNA+Polymerase/pmc13635745-73-5-9
    Average 99 stars, based on 9986 article reviews
    q5 high fidelity dna polymerase - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Sequencing:

    Article Title: DCTPP1 orchestrates dCTP pool dynamics and mtDNA stability in quiescent cells.
    Article Snippet: Flow cytometry data were processed and FlowJo 7.6 software (Ashland, OR, USA) was used for cell cycle analysis. .. AR TI CL E IN P RE SS dNTP pools quantification dNTPs pool determination was performed using the DNA polymerase assay (13), based on the incorporation of [3H]-dATP (Hartman Analytic, Braunschweig, Deutschland; 16.8 Ci/mmol) or [3H]-dTTP (Perkin-Elmer Life Sciences; 20.7 Ci/mmol) into sequence-specific oligonucleotides by a DNA polymerase (E.coli Klenow fragment, New England Biolabs Ipswich, MA,USA). ..

    Hybridization:

    Article Title: sc-rDSeq: a robust and cost-effective full-length total RNA sequencing method for single cells reveals multilayered heterogeneity in drug-resistant lung cancer cells
    Article Snippet: .. After denaturation at 85°C for 2 min and hybridization at 60°C for 20 min, 5 μl of primer extension mix containing 1.8 U of DNA Polymerase (NEB, cat #M0537) and 0.3 mM dNTP (NEB, cat #N0446) in 1× isothermal amplification buffer (NEB, cat #B0537) was added, followed by incubation at 60°C for 60 min. ..

    Amplification:

    Article Title: sc-rDSeq: a robust and cost-effective full-length total RNA sequencing method for single cells reveals multilayered heterogeneity in drug-resistant lung cancer cells
    Article Snippet: .. After denaturation at 85°C for 2 min and hybridization at 60°C for 20 min, 5 μl of primer extension mix containing 1.8 U of DNA Polymerase (NEB, cat #M0537) and 0.3 mM dNTP (NEB, cat #N0446) in 1× isothermal amplification buffer (NEB, cat #B0537) was added, followed by incubation at 60°C for 60 min. ..

    Article Title: Methods and systems for droplet-based single cell barcoding
    Article Snippet: .. At (i) in FIG. 21, a DNA polymerase, such as phi29 DNA Polymerase (New England Biolabs® Inc. (NEB), Ipswich, MA), may be used to perform isothermal amplification. ..

    Incubation:

    Article Title: sc-rDSeq: a robust and cost-effective full-length total RNA sequencing method for single cells reveals multilayered heterogeneity in drug-resistant lung cancer cells
    Article Snippet: .. After denaturation at 85°C for 2 min and hybridization at 60°C for 20 min, 5 μl of primer extension mix containing 1.8 U of DNA Polymerase (NEB, cat #M0537) and 0.3 mM dNTP (NEB, cat #N0446) in 1× isothermal amplification buffer (NEB, cat #B0537) was added, followed by incubation at 60°C for 60 min. ..

    cDNA Synthesis:

    Article Title: Comparative effects of bile salts and free bile acids on intestinal health of grouper ( Epinephelus spp.) fed a high cottonseed protein concentrate diet
    Article Snippet: First-strand cDNA synthesis was conducted with random primers and Superscript II Reverse Transcriptase (catalog no. 18064014, Invitrogen, Carlsbad, CA, USA). .. Subsequently, second-strand cDNA synthesis was performed, employing DNA Polymerase I and RNase H provided in the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (catalog no. E7760S/L, New England Biolabs, Ipswich, MA, USA). ..

    Article Title: PVT1‐104aa derived from the 8q24 gene desert promotes colorectal cancer tumorigenesis
    Article Snippet: Library generation was then conducted applying the TruSeq Stranded Total RNA Library Prep System (Illumina, Cat. No. 20020599). .. Briefly, the enriched RNA was fragmented using divalent cations under elevated temperature (94°C). cDNA synthesis was initiated with SuperScript IV Reverse Transcriptase (Thermo Fisher Scientific, Cat. No. 18090010) and random hexamers in the first step, followed by second‐strand generation using DNA Polymerase I and RNase H (NEBNext Second Strand Synthesis Module, NEB, Cat. No. E6111). .. The cDNA fragments underwent end repair and A‐tailing (NEBNext Ultra II End Repair/dA‐Tailing Module, NEB, Cat. No. E7546), followed by adapter ligation (TruSeq RNA UD Indexes, Illumina, Cat. No. 20022371).

    Reverse Transcription:

    Article Title: PVT1‐104aa derived from the 8q24 gene desert promotes colorectal cancer tumorigenesis
    Article Snippet: Library generation was then conducted applying the TruSeq Stranded Total RNA Library Prep System (Illumina, Cat. No. 20020599). .. Briefly, the enriched RNA was fragmented using divalent cations under elevated temperature (94°C). cDNA synthesis was initiated with SuperScript IV Reverse Transcriptase (Thermo Fisher Scientific, Cat. No. 18090010) and random hexamers in the first step, followed by second‐strand generation using DNA Polymerase I and RNase H (NEBNext Second Strand Synthesis Module, NEB, Cat. No. E6111). .. The cDNA fragments underwent end repair and A‐tailing (NEBNext Ultra II End Repair/dA‐Tailing Module, NEB, Cat. No. E7546), followed by adapter ligation (TruSeq RNA UD Indexes, Illumina, Cat. No. 20022371).

    Polymerase Chain Reaction:

    Article Title: Comparison of cell isolates within anatomical regions of the ovine umbilical cord for improved isolation of putative mesenchymal stem cells.
    Article Snippet: .. PCR reactions of 25 μl were prepared containing final concentrations of 1 x β-2-microglobulin qPCR primer-probe assay (Table 1), 0.2 mM each dNTPs (Thermo Fisher Scientific, Waltham, USA, Invitrogen Cat No. 10297018), 1.5 mM MgCl2 and 1.5 units taq DNA polymerase (New England Biolabs, Ipswich, USA, Cat No. M0320S) along with 1 ng of the synthesized cDNA. .. Amplification was performed using a T100TM Thermal Cycler (Bio-Rad, Hercules, USA) with initial denaturation at 94 ◦C for 1 min; 34 cycles of 94 ◦C for 30 s, 55 ◦C for 30 s, 72 ◦C for 30 s; followed by a final extension step at 72 ◦C for 7 min. PCR products (10 μl) along with 2.5 μl of 1 Kb Plus DNA Ladder at each end (Thermo Fisher Scientific, Waltham, USA, Cat. No. 10787018) were resolved by electrophoresis on 3% agarose gels, prepared by dissolving AxygenTM Agarose LE (Thermo Fisher Scientific, Waltham, USA, Cat. No. 14223081) and 0.5 μl Gel Red dye (Biotium, Fremont, USA, Cat. No. 41003) in 1 x Tris-Boric acid-EDTA buffer (Merck KGaA, Darmstadt, Germany, Sigma Aldrich Cat. No's T1503-1 KG, B0252-1 KG, 10425S) run at 100 V for 45 min and imaged for the expected 119 bp amplicon using a Bio-Rad Chemidoc MP (Bio-Rad, Hercules, USA).

    Real-time Polymerase Chain Reaction:

    Article Title: Comparison of cell isolates within anatomical regions of the ovine umbilical cord for improved isolation of putative mesenchymal stem cells.
    Article Snippet: .. PCR reactions of 25 μl were prepared containing final concentrations of 1 x β-2-microglobulin qPCR primer-probe assay (Table 1), 0.2 mM each dNTPs (Thermo Fisher Scientific, Waltham, USA, Invitrogen Cat No. 10297018), 1.5 mM MgCl2 and 1.5 units taq DNA polymerase (New England Biolabs, Ipswich, USA, Cat No. M0320S) along with 1 ng of the synthesized cDNA. .. Amplification was performed using a T100TM Thermal Cycler (Bio-Rad, Hercules, USA) with initial denaturation at 94 ◦C for 1 min; 34 cycles of 94 ◦C for 30 s, 55 ◦C for 30 s, 72 ◦C for 30 s; followed by a final extension step at 72 ◦C for 7 min. PCR products (10 μl) along with 2.5 μl of 1 Kb Plus DNA Ladder at each end (Thermo Fisher Scientific, Waltham, USA, Cat. No. 10787018) were resolved by electrophoresis on 3% agarose gels, prepared by dissolving AxygenTM Agarose LE (Thermo Fisher Scientific, Waltham, USA, Cat. No. 14223081) and 0.5 μl Gel Red dye (Biotium, Fremont, USA, Cat. No. 41003) in 1 x Tris-Boric acid-EDTA buffer (Merck KGaA, Darmstadt, Germany, Sigma Aldrich Cat. No's T1503-1 KG, B0252-1 KG, 10425S) run at 100 V for 45 min and imaged for the expected 119 bp amplicon using a Bio-Rad Chemidoc MP (Bio-Rad, Hercules, USA).

    Synthesized:

    Article Title: Comparison of cell isolates within anatomical regions of the ovine umbilical cord for improved isolation of putative mesenchymal stem cells.
    Article Snippet: .. PCR reactions of 25 μl were prepared containing final concentrations of 1 x β-2-microglobulin qPCR primer-probe assay (Table 1), 0.2 mM each dNTPs (Thermo Fisher Scientific, Waltham, USA, Invitrogen Cat No. 10297018), 1.5 mM MgCl2 and 1.5 units taq DNA polymerase (New England Biolabs, Ipswich, USA, Cat No. M0320S) along with 1 ng of the synthesized cDNA. .. Amplification was performed using a T100TM Thermal Cycler (Bio-Rad, Hercules, USA) with initial denaturation at 94 ◦C for 1 min; 34 cycles of 94 ◦C for 30 s, 55 ◦C for 30 s, 72 ◦C for 30 s; followed by a final extension step at 72 ◦C for 7 min. PCR products (10 μl) along with 2.5 μl of 1 Kb Plus DNA Ladder at each end (Thermo Fisher Scientific, Waltham, USA, Cat. No. 10787018) were resolved by electrophoresis on 3% agarose gels, prepared by dissolving AxygenTM Agarose LE (Thermo Fisher Scientific, Waltham, USA, Cat. No. 14223081) and 0.5 μl Gel Red dye (Biotium, Fremont, USA, Cat. No. 41003) in 1 x Tris-Boric acid-EDTA buffer (Merck KGaA, Darmstadt, Germany, Sigma Aldrich Cat. No's T1503-1 KG, B0252-1 KG, 10425S) run at 100 V for 45 min and imaged for the expected 119 bp amplicon using a Bio-Rad Chemidoc MP (Bio-Rad, Hercules, USA).



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    Image Search Results


    The reaction principle and workflow of the CRISPR‐Scan system. (a) Workflow of the CRISPR‐Scan detection system. Genomic DNA was extracted from patient blood samples, and 1 µL of genomic DNA was directly added to the CRISPR‐Scan reaction mixture, which contained T7 RNA polymerase, RPA reagents, a pair of primers, RNA reporter, LwaCas13a, and a single crRNA. The one‐pot reaction was performed at 39°C for 30 min inside the homemade microfluidic device. Real‐time fluorescence curves and visual detection results were recorded with a smartphone, and detection results were output automatically. (b) For CRISPR‐Scan detection, the mutant or wild‐type (WT) template was amplified using primers containing a T7 promoter sequence by RPA, followed by T7 transcription to produce RNA products. LwaCas13a/crRNA recognized RNA targets with 1–4 bp mismatches, activated its trans ‐cleavage activity, and induced cleavage of the RNA reporter, which produces fluorescence signals. However, the WT RNA contains a large number of base mismatches, making it difficult for LwaCas13a/crRNA to recognize, and therefore no fluorescence signals were generated. LwaCas13a/crRNA can achieve the distinction between WT and NPM1 gene c.863_864 4‐bp insertions, while also covering the detection of 4‐bp random insertion mutations. Created within BioRender.com. Bai, L. (2026) https://BioRender.com/12nctes .

    Journal: Advanced Science

    Article Title: CRISPR‐Scan: Cas13a Mismatch Tolerance Enabled Ultra‐Multiplexed Detection of Same‐Site Indels in Acute Myeloid Leukemia

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    Figure Lengend Snippet: The reaction principle and workflow of the CRISPR‐Scan system. (a) Workflow of the CRISPR‐Scan detection system. Genomic DNA was extracted from patient blood samples, and 1 µL of genomic DNA was directly added to the CRISPR‐Scan reaction mixture, which contained T7 RNA polymerase, RPA reagents, a pair of primers, RNA reporter, LwaCas13a, and a single crRNA. The one‐pot reaction was performed at 39°C for 30 min inside the homemade microfluidic device. Real‐time fluorescence curves and visual detection results were recorded with a smartphone, and detection results were output automatically. (b) For CRISPR‐Scan detection, the mutant or wild‐type (WT) template was amplified using primers containing a T7 promoter sequence by RPA, followed by T7 transcription to produce RNA products. LwaCas13a/crRNA recognized RNA targets with 1–4 bp mismatches, activated its trans ‐cleavage activity, and induced cleavage of the RNA reporter, which produces fluorescence signals. However, the WT RNA contains a large number of base mismatches, making it difficult for LwaCas13a/crRNA to recognize, and therefore no fluorescence signals were generated. LwaCas13a/crRNA can achieve the distinction between WT and NPM1 gene c.863_864 4‐bp insertions, while also covering the detection of 4‐bp random insertion mutations. Created within BioRender.com. Bai, L. (2026) https://BioRender.com/12nctes .

    Article Snippet: Recombinant RNase Inhibitor (Cat. No. 2313A) and Taq DNA Polymerase (Cat. No. R007A) were purchased from Takara Bio (Dalian, China).

    Techniques: CRISPR, Blood/Plasma/Serum Samples, Fluorescence, Mutagenesis, Amplification, Sequencing, Activity Assay, Generated

    Journal: iScience

    Article Title: Gapmers targeting conserved SARS-CoV-2 single-stranded regions reduce viral load and reshape inflammatory macrophage responses

    doi: 10.1016/j.isci.2026.117639

    Figure Lengend Snippet:

    Article Snippet: OneTaq DNA Polymerase , NEB , M0480.

    Techniques: Virus, Variant Assay, Recombinant, Reverse Transcription, Diagnostic Assay, Luminex, Ligation, Multiplex Assay, Generated, Software